Genetic consequences of ICSI: Micro-assisted fertilization and sperm chromosome abnormalities
نویسندگان
چکیده
منابع مشابه
Safety issues in assisted reproduction technology: Should men undergoing ICSI be screened for chromosome abnormalities in their sperm?
The incidence of aneuploidy in gametes of men undergoing ICSI has raised the prospect of there being risks associated with ICSI and the question of whether or not to screen men for sperm aneuploidy before treatment. We report results of a questionnaire undertaken to address how IVF staff perceive this problem, whether ICSI men are already being screened for sperm aneuploidy and the extent to wh...
متن کاملTreatment option for sperm- or oocyte-related fertilization failure: assisted oocyte activation following diagnostic heterologous ICSI.
BACKGROUND Failed fertilization occurs in 2-3% of ICSI cycles and is mainly due to lack of oocyte activation. Heterologous ICSI of patient's sperm in mouse oocytes allows discrimination between sperm- and oocyte-related aetiologies of activation failure. Assisted oocyte activation (AOA) by Ca-ionophore treatment can initiate fertilization in subsequent therapeutic ICSI. We report on diagnosis a...
متن کاملEffect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSI.
The aim of this study was to evaluate the effect of sperm DNA damage and protamine deficiency on fertilization and embryo development post-intracytoplasmic sperm injection (ICSI), and also to assess the effect of protamine deficiency on DNA damage. Semen samples were collected from 28 patients participating in the ICSI programme. Following sperm preparation and ICSI, the remaining processed sem...
متن کاملEffect of Protamine Deficiency on Fertilization Outcome and Incidence of Sperm Premature Chromosomal Condensation Post ICSI
Purpose: After aneuploidy,sperm premature chromatin condensation (PCC) is the next most important cause of fertilization failure. Therefore the aim of this study is to evaluate the effect of sperm protamine deficiency on sperm PCC formation post ICSI. Materials and Methods: Cytogenetic analysis was carried out on unfertilized oocytes post ICSI and incidence of sperm PCC was evaluated. The resu...
متن کاملIncidence of sperm chromosomal abnormalities in a risk population: relationship with sperm quality and ICSI outcome.
BACKGROUND An increased incidence of chromosome abnormalities has been reported in sperm samples of many infertile men by fluorescence in-situ hybridization (FISH). METHODS Sperm aneuploidy and diploidy rates for chromosomes 13, 18, 21, X and Y were evaluated in 63 patients with normal karyotypes using dual and triple-colour FISH techniques. Indications for sperm FISH analysis were: recurrent...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1996
ISSN: 0268-1161,1460-2350
DOI: 10.1093/oxfordjournals.humrep.a019322